Genotyping and primer details


Genotyping and primer details for 396G05 - At4g25320
Line ID 396G05
Locus At4g25320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12955256 of line 396G05 Chr4:12955256
   
Insertion allele amplicon 8474 - 29qv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29qv: 5'-ATGGCTCAAGAAGTCTCAAATGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12954889
   
wt amplicon (over InsPos) 03rj - 29qv
wt amplicon start Chr4:12954889
wt amplicon end Chr4:12955581
wt amplicon size 693 bp
Locus specific primer 29qv: 5'-ATGGCTCAAGAAGTCTCAAATGTT-3'
2nd locus specific primer 03rj: 5'-GATGTCATAGATTGACGAAGCGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11