Genotyping and primer details


Genotyping and primer details for 396H05 - At2g23140
Line ID 396H05
Locus At2g23140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9845738 of line 396H05 Chr2:9845738
   
Insertion allele amplicon 8474 - 07ht
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07ht: 5'-ATCTTATTGATCTGATGGACCCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9846160
   
wt amplicon (over InsPos) 06io - 07ht
wt amplicon start Chr2:9845153
wt amplicon end Chr2:9846160
wt amplicon size 1008 bp
Locus specific primer 07ht: 5'-ATCTTATTGATCTGATGGACCCAG-3'
2nd locus specific primer 06io: 5'-AATCGCATGGTCTCTACTTGTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11