Genotyping and primer details


Genotyping and primer details for 399C06 - At2g20620
Line ID 399C06
Locus At2g20620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8892970 of line 399C06 Chr2:8892970
   
Insertion allele amplicon 8474 - CB52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CB52: 5'-AGTTATACCTTCAAGGCTGCAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8892614
   
wt amplicon (over InsPos) 10ah - CB52
wt amplicon start Chr2:8892614
wt amplicon end Chr2:8893582
wt amplicon size 969 bp
Locus specific primer CB52: 5'-AGTTATACCTTCAAGGCTGCAAAA-3'
2nd locus specific primer 10ah: 5'-AATTTAAAACAATGTTGTCCCTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11