Genotyping and primer details


Genotyping and primer details for 400H09 - At5g20290
Line ID 400H09
Locus At5g20290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6852291 of line 400H09 Chr5:6852291
   
Insertion allele amplicon 8474 - LB13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer LB13: 5'-CGAAAGTGAGTCTTTCGTTTTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6852828
   
wt amplicon (over InsPos) 03li - LB13
wt amplicon start Chr5:6851660
wt amplicon end Chr5:6852828
wt amplicon size 1169 bp
Locus specific primer LB13: 5'-CGAAAGTGAGTCTTTCGTTTTTCT-3'
2nd locus specific primer 03li: 5'-GTAAGACAAAAGCTCAAGGCAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11