Genotyping and primer details


Genotyping and primer details for 401D10 - At3g16870
Line ID 401D10
Locus At3g16870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5763767 of line 401D10 Chr3:5763767
   
Insertion allele amplicon 8474 - 47vd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 47vd: 5'-AATTTAGTGACCGGAAAGATCTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5764220
   
wt amplicon (over InsPos) 44vn - 47vd
wt amplicon start Chr3:5763714
wt amplicon end Chr3:5764220
wt amplicon size 507 bp
Locus specific primer 47vd: 5'-AATTTAGTGACCGGAAAGATCTGA-3'
2nd locus specific primer 44vn: 5'-TCGCTCTCACTTGATTATGACCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11