Genotyping and primer details


Genotyping and primer details for 401D12 - At2g39480
Line ID 401D12
Locus At2g39480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16482089 of line 401D12 Chr2:16482089
   
Insertion allele amplicon 8474 - JV62
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JV62: 5'-AAGTGAGCTGATAAATGTGTGTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16481506
   
wt amplicon (over InsPos) 05hn - JV62
wt amplicon start Chr2:16481506
wt amplicon end Chr2:16482253
wt amplicon size 748 bp
Locus specific primer JV62: 5'-AAGTGAGCTGATAAATGTGTGTGC-3'
2nd locus specific primer 05hn: 5'-TTTTTGCTGTTATTTTAAGTGGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11