Genotyping and primer details


Genotyping and primer details for 403D11 - At3g58110
Line ID 403D11
Locus At3g58110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21518197 of line 403D11 Chr3:21518197
   
Insertion allele amplicon 8474 - 13wm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13wm: 5'-GCAAATGACAATCTAGCTTTTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21518558
   
wt amplicon (over InsPos) 84ww - 13wm
wt amplicon start Chr3:21517442
wt amplicon end Chr3:21518558
wt amplicon size 1117 bp
Locus specific primer 13wm: 5'-GCAAATGACAATCTAGCTTTTTCC-3'
2nd locus specific primer 84ww: 5'-ACGATGAATCGGTTAGGTTTATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11