Genotyping and primer details


Genotyping and primer details for 405D02 - At1g77630
Line ID 405D02
Locus At1g77630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29175328 of line 405D02 Chr1:29175328
   
Insertion allele amplicon 8474 - 29cp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29cp: 5'-ACTTGTAGTTACAACGGTTTCGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29174827
   
wt amplicon (over InsPos) 20dk - 29cp
wt amplicon start Chr1:29174827
wt amplicon end Chr1:29175902
wt amplicon size 1076 bp
Locus specific primer 29cp: 5'-ACTTGTAGTTACAACGGTTTCGCT-3'
2nd locus specific primer 20dk: 5'-GAGCGTCTTTCTAGGTTGAAGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11