Genotyping and primer details


Genotyping and primer details for 405E02 - At1g78890
Line ID 405E02
Locus At1g78890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29657201 of line 405E02 Chr1:29657201
   
Insertion allele amplicon 8474 - CB64
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CB64: 5'-CTCGGAGTGATATGCAGTATTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29657525
   
Insertion allele amplicon 8474 - CB65
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CB65: 5'-TTGTCAATAAGTTGGAAGATGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29656795
   
wt amplicon (over InsPos) CB65 - CB64
wt amplicon start Chr1:29656795
wt amplicon end Chr1:29657525
wt amplicon size 731 bp
Locus specific primer CB64: 5'-CTCGGAGTGATATGCAGTATTTGA-3'
2nd locus specific primer CB65: 5'-TTGTCAATAAGTTGGAAGATGCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11