Genotyping and primer details


Genotyping and primer details for 407B03 - At1g71490
Line ID 407B03
Locus At1g71490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26934164 of line 407B03 Chr1:26934164
   
Insertion allele amplicon 8474 - 16de
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16de: 5'-TTTAGAAATATGGGTATTGCTCGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26934798
   
wt amplicon (over InsPos) 53dy - 16de
wt amplicon start Chr1:26933841
wt amplicon end Chr1:26934798
wt amplicon size 958 bp
Locus specific primer 16de: 5'-TTTAGAAATATGGGTATTGCTCGG-3'
2nd locus specific primer 53dy: 5'-ACTGCATCTTCATAAACAGCCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11