Genotyping and primer details


Genotyping and primer details for 407C06 - At3g56030
Line ID 407C06
Locus At3g56030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20791982 of line 407C06 Chr3:20791982
   
Insertion allele amplicon 8474 - 13wu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13wu: 5'-AATTTGCTGTAAATGTTTGCTCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20792599
   
wt amplicon (over InsPos) 06xf - 13wu
wt amplicon start Chr3:20791521
wt amplicon end Chr3:20792599
wt amplicon size 1079 bp
Locus specific primer 13wu: 5'-AATTTGCTGTAAATGTTTGCTCCT-3'
2nd locus specific primer 06xf: 5'-GTTTCAACACGACAGCTACTTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11