Genotyping and primer details


Genotyping and primer details for 408D03 - At5g16500
Line ID 408D03
Locus At5g16500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5388865 of line 408D03 Chr5:5388865
   
Insertion allele amplicon 8474 - 14wm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14wm: 5'-AACAACTTCAAAGAAAATCTCCGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5388534
   
wt amplicon (over InsPos) 83ww - 14wm
wt amplicon start Chr5:5388534
wt amplicon end Chr5:5389664
wt amplicon size 1131 bp
Locus specific primer 14wm: 5'-AACAACTTCAAAGAAAATCTCCGA-3'
2nd locus specific primer 83ww: 5'-AGATGTTAAGTGTCCAACCAATCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11