Genotyping and primer details


Genotyping and primer details for 413A11 - At5g15510
Line ID 413A11
Locus At5g15510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5035595 of line 413A11 Chr5:5035595
   
Insertion allele amplicon 8474 - 14wu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14wu: 5'-CACTATCAGCTATTTTCCTTTCCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5035922
   
wt amplicon (over InsPos) 24xd - 14wu
wt amplicon start Chr5:5034275
wt amplicon end Chr5:5035922
wt amplicon size 1648 bp
Locus specific primer 14wu: 5'-CACTATCAGCTATTTTCCTTTCCG-3'
2nd locus specific primer 24xd: 5'-GAAGAAATCAGCGCAGAAGAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11