Genotyping and primer details


Genotyping and primer details for 413B06 - At3g26490
Line ID 413B06
Locus At3g26490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9704788 of line 413B06 Chr3:9704788
   
Insertion allele amplicon 8474 - 18us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18us: 5'-CCAAGTCAAATCCACAAAATACCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9704334
   
wt amplicon (over InsPos) 44vc - 18us
wt amplicon start Chr3:9704334
wt amplicon end Chr3:9705335
wt amplicon size 1002 bp
Locus specific primer 18us: 5'-CCAAGTCAAATCCACAAAATACCT-3'
2nd locus specific primer 44vc: 5'-ATAAGGGTATCAACAGCTCGCTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11