Genotyping and primer details


Genotyping and primer details for 413F08 - At4g08310
Line ID 413F08
Locus At4g08310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:5249221 of line 413F08 Chr4:5249221
   
Insertion allele amplicon 8474 - CE05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CE05: 5'-ATTATTCGATCAAAGACGGAATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5248793
   
Insertion allele amplicon 8474 - CE06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CE06: 5'-TCTTTCTTGGCAGTATTTCTCCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5249707
   
wt amplicon (over InsPos) CE06 - CE05
wt amplicon start Chr4:5248793
wt amplicon end Chr4:5249707
wt amplicon size 915 bp
Locus specific primer CE05: 5'-ATTATTCGATCAAAGACGGAATTG-3'
2nd locus specific primer CE06: 5'-TCTTTCTTGGCAGTATTTCTCCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11