Genotyping and primer details


Genotyping and primer details for 414D11 - At2g28990
Line ID 414D11
Locus At2g28990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12455314 of line 414D11 Chr2:12455314
   
Insertion allele amplicon 8474 - 24wq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 24wq: 5'-GTTTGATTTGGTTGTTTGGTTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12456165
   
Insertion allele amplicon 8474 - EQ58
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ58: 5'-AGATCATCTGGCGATAAACCATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12454792
   
wt amplicon (over InsPos) EQ59 - EQ58
wt amplicon start Chr2:12454792
wt amplicon end Chr2:12455668
wt amplicon size 877 bp
Locus specific primer EQ58: 5'-AGATCATCTGGCGATAAACCATAC-3'
2nd locus specific primer EQ59: 5'-TTATTGGCAAAGTTTCTCCTGTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11