Genotyping and primer details


Genotyping and primer details for 414E07 - At5g57440
Line ID 414E07
Locus At5g57440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23272523 of line 414E07 Chr5:23272523
   
Insertion allele amplicon 8474 - CC13
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CC13: 5'-GGAGACTGTTGCCTGTTAGTTACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23271881
   
Insertion allele amplicon 8474 - CC14
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CC14: 5'-AACTAACTTCCGACTGAAACTGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23272956
   
wt amplicon (over InsPos) CC14 - CC13
wt amplicon start Chr5:23271881
wt amplicon end Chr5:23272956
wt amplicon size 1076 bp
Locus specific primer CC13: 5'-GGAGACTGTTGCCTGTTAGTTACC-3'
2nd locus specific primer CC14: 5'-AACTAACTTCCGACTGAAACTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11