Genotyping and primer details


Genotyping and primer details for 416C06 - At5g57410
Line ID 416C06
Locus At5g57410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23265255 of line 416C06 Chr5:23265255
   
Insertion allele amplicon 8474 - 32tv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32tv: 5'-AAGGAGAGTTTTGGTGGTAGTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23265001
   
wt amplicon (over InsPos) 09uu - 32tv
wt amplicon start Chr5:23265001
wt amplicon end Chr5:23265578
wt amplicon size 578 bp
Locus specific primer 32tv: 5'-AAGGAGAGTTTTGGTGGTAGTGAA-3'
2nd locus specific primer 09uu: 5'-AGCAACAGAGAGAGAGCTTGAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11