Genotyping and primer details


Genotyping and primer details for 417B05 - At1g62520
Line ID 417B05
Locus At1g62520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23144694 of line 417B05 Chr1:23144694
   
Insertion allele amplicon 8474 - AB69
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB69: 5'-ATTATGAGAAAACAAGGCAACACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23145335
   
Insertion allele amplicon 8474 - AR05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AR05: 5'-TAAAAACAATAAAATCCGGCTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23144090
   
wt amplicon (over InsPos) AR05 - AB69
wt amplicon start Chr1:23144090
wt amplicon end Chr1:23145335
wt amplicon size 1246 bp
Locus specific primer AB69: 5'-ATTATGAGAAAACAAGGCAACACC-3'
2nd locus specific primer AR05: 5'-TAAAAACAATAAAATCCGGCTGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11