Genotyping and primer details


Genotyping and primer details for 419E07 - At3g15570
Line ID 419E07
Locus At3g15570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5271679 of line 419E07 Chr3:5271679
   
Insertion allele amplicon 8474 - 50ns
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 50ns: 5'-ATACTCTGGTTTCAAGCTCCGTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5271238
   
wt amplicon (over InsPos) 25oj - 50ns
wt amplicon start Chr3:5271238
wt amplicon end Chr3:5272001
wt amplicon size 764 bp
Locus specific primer 50ns: 5'-ATACTCTGGTTTCAAGCTCCGTTA-3'
2nd locus specific primer 25oj: 5'-TTACGATTTGATTGTCGGTCACTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11