Genotyping and primer details


Genotyping and primer details for 424B11 - At1g48970
Line ID 424B11
Locus At1g48970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18116808 of line 424B11 Chr1:18116808
   
Insertion allele amplicon 8474 - 21us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21us: 5'-CTAATGGAGGCTTTGATTTGACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18117096
   
wt amplicon (over InsPos) 49wd - 21us
wt amplicon start Chr1:18115985
wt amplicon end Chr1:18117096
wt amplicon size 1112 bp
Locus specific primer 21us: 5'-CTAATGGAGGCTTTGATTTGACTC-3'
2nd locus specific primer 49wd: 5'-AGCTTAATAACCCCTCCAGAGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11