Genotyping and primer details


Genotyping and primer details for 424C01 - At2g45290
Line ID 424C01
Locus At2g45290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18673324 of line 424C01 Chr2:18673324
   
Insertion allele amplicon 8474 - 23qw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23qw: 5'-AAGAAAGATAACAATAAAAGTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18673733
   
wt amplicon (over InsPos) 04rm - 23qw
wt amplicon start Chr2:18672942
wt amplicon end Chr2:18673733
wt amplicon size 792 bp
Locus specific primer 23qw: 5'-AAGAAAGATAACAATAAAAGTGC-3'
2nd locus specific primer 04rm: 5'-TAGAGGCAATCGTGACAAGTTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11