Genotyping and primer details


Genotyping and primer details for 425D06 - At5g48180
Line ID 425D06
Locus At5g48180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19542376 of line 425D06 Chr5:19542376
   
Insertion allele amplicon 8474 - 25fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25fr: 5'-CTTTAGCAGTAACACCACCAAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19541831
   
wt amplicon (over InsPos) 12gl - 25fr
wt amplicon start Chr3:2362662
wt amplicon end Chr5:19541831
wt amplicon size 1002 bp
Locus specific primer 25fr: 5'-CTTTAGCAGTAACACCACCAAACA-3'
2nd locus specific primer 12gl: 5'-GTTTGGACGATTTTACCCTTGATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11