Genotyping and primer details


Genotyping and primer details for 425G12 - At4g16100
Line ID 425G12
Locus At4g16100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9105896 of line 425G12 Chr4:9105896
   
Insertion allele amplicon 8474 - KP41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KP41: 5'-GGGTTTCCTCAATTGTATGAGCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9104979
   
wt amplicon (over InsPos) 31xo - KP41
wt amplicon start Chr4:9104979
wt amplicon end Chr4:9106456
wt amplicon size 1478 bp
Locus specific primer KP41: 5'-GGGTTTCCTCAATTGTATGAGCTA-3'
2nd locus specific primer 31xo: 5'-AGCAAGAAATAGGGACGGTACTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11