Genotyping and primer details


Genotyping and primer details for 426C05 - At2g28500
Line ID 426C05
Locus At2g28500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12187015 of line 426C05 Chr2:12187015
   
Insertion allele amplicon 8474 - 47pv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 47pv: 5'-GCAGTTTCGTTACGTGCTTATACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12186450
   
wt amplicon (over InsPos) 03tx - 47pv
wt amplicon start Chr2:12186450
wt amplicon end Chr2:12187376
wt amplicon size 927 bp
Locus specific primer 47pv: 5'-GCAGTTTCGTTACGTGCTTATACA-3'
2nd locus specific primer 03tx: 5'-GGTCAATAACAACCTTTCCTCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11