Genotyping and primer details


Genotyping and primer details for 427C03 - At5g58870
Line ID 427C03
Locus At5g58870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23770837 of line 427C03 Chr5:23770837
   
Insertion allele amplicon 8474 - BC05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BC05: 5'-GAGTCTAGTGACCATATCATGCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23770288
   
wt amplicon (over InsPos) 18fo - BC05
wt amplicon start Chr5:23770288
wt amplicon end Chr5:23771320
wt amplicon size 1033 bp
Locus specific primer BC05: 5'-GAGTCTAGTGACCATATCATGCCA-3'
2nd locus specific primer 18fo: 5'-AGGAAGAGCAAGATGACTGTTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11