Genotyping and primer details


Genotyping and primer details for 429H05 - At1g36280
Line ID 429H05
Locus At1g36280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:13642637 of line 429H05 Chr1:13642637
   
Insertion allele amplicon 8474 - 55ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 55ra: 5'-AACAAGCAGAATCATCTACACCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13642945
   
wt amplicon (over InsPos) 35rt - 55ra
wt amplicon start Chr1:13642400
wt amplicon end Chr1:13642945
wt amplicon size 546 bp
Locus specific primer 55ra: 5'-AACAAGCAGAATCATCTACACCAA-3'
2nd locus specific primer 35rt: 5'-GAGCTTTAGGACACTCTCTTCTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11