Genotyping and primer details


Genotyping and primer details for 430G11 - At2g41730
Line ID 430G11
Locus At2g41730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17409653 of line 430G11 Chr2:17409653
   
Insertion allele amplicon 8474 - CB31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CB31: 5'-GACTTTAAGATTTGTAACCGCGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17410209
   
wt amplicon (over InsPos) 19rd - CB31
wt amplicon start Chr2:17409248
wt amplicon end Chr2:17410209
wt amplicon size 962 bp
Locus specific primer CB31: 5'-GACTTTAAGATTTGTAACCGCGAG-3'
2nd locus specific primer 19rd: 5'-AGTCCACAAAATTAGGAAGAGCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11