Genotyping and primer details


Genotyping and primer details for 433D08 - At5g54040
Line ID 433D08
Locus At5g54040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21929744 of line 433D08 Chr5:21929744
   
Insertion allele amplicon 8474 - 40xt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 40xt: 5'-GATGTACTTCCATCCTAGGACCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21929050
   
Insertion allele amplicon 8474 - 47xw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 47xw: 5'-TTTATATTAATTAGCCGGCAGTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21930809
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11