Genotyping and primer details


Genotyping and primer details for 438C06 - At4g04790
Line ID 438C06
Locus At4g04790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:2435010 of line 438C06 Chr4:2435010
   
Insertion allele amplicon 8474 - 41vw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 41vw: 5'-TTCTCGGTCCCAAACAATATTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:2434278
   
Insertion allele amplicon 8474 - 51wp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 51wp: 5'-AGCTCGAGTGATCGAGTCTGTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:2435762
   
wt amplicon (over InsPos) 41vw - 22ve
wt amplicon start Chr4:2434278
wt amplicon end Chr4:2435505
wt amplicon size 1228 bp
Locus specific primer 22ve: 5'-ATTGGTGTGGAAAGAGTACCAATC-3'
2nd locus specific primer 41vw: 5'-TTCTCGGTCCCAAACAATATTAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11