Genotyping and primer details


Genotyping and primer details for 438H03 - At4g33270
Line ID 438H03
Locus At4g33270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16044602 of line 438H03 Chr4:16044602
   
Insertion allele amplicon 8474 - 29lf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29lf: 5'-GTTAGGTTATTGTGTGATTGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16043964
   
Insertion allele amplicon 8474 - JL17
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JL17: 5'-TGTGTGATCGGTATTTTTCACCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16044015
   
Insertion allele amplicon 8474 - JL18
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JL18: 5'-CAGTTGGACTCAACAACTCTGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16042187
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11