Genotyping and primer details


Genotyping and primer details for 440H12 - At3g46590
Line ID 440H12
Locus At3g46590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17156164 of line 440H12 Chr3:17156164
   
Insertion allele amplicon 8474 - 58ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 58ra: 5'-CACTTTCTCTTCTTCATGTTGCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17155591
   
wt amplicon (over InsPos) 33rt - 58ra
wt amplicon start Chr3:17155591
wt amplicon end Chr3:17156298
wt amplicon size 708 bp
Locus specific primer 58ra: 5'-CACTTTCTCTTCTTCATGTTGCTG-3'
2nd locus specific primer 33rt: 5'-AACTGACCAAACATAGACCAGCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11