Genotyping and primer details


Genotyping and primer details for 441E03 - At5g61240
Line ID 441E03
Locus At5g61240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24631212 of line 441E03 Chr5:24631212
   
Insertion allele amplicon 8474 - 42by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 42by: 5'-AAACTCAGGATCCAATTCTCTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24630540
   
wt amplicon (over InsPos) 04dk - 42by
wt amplicon start Chr5:24630540
wt amplicon end Chr5:24631504
wt amplicon size 965 bp
Locus specific primer 42by: 5'-AAACTCAGGATCCAATTCTCTTCA-3'
2nd locus specific primer 04dk: 5'-TCAGCGATCTAGCACAAAGACTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11