Genotyping and primer details


Genotyping and primer details for 449E05 - At5g10210
Line ID 449E05
Locus At5g10210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3206481 of line 449E05 Chr5:3206481
   
Insertion allele amplicon 8474 - JH19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JH19: 5'-TCACAAAGGAACTCACTGCTCTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3206144
   
wt amplicon (over InsPos) 06ua - JH19
wt amplicon start Chr5:3206144
wt amplicon end Chr5:3206818
wt amplicon size 675 bp
Locus specific primer JH19: 5'-TCACAAAGGAACTCACTGCTCTTA-3'
2nd locus specific primer 06ua: 5'-CTAACGCACAAGCTACAACAAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11