Genotyping and primer details


Genotyping and primer details for 449G02 - At3g50240
Line ID 449G02
Locus At3g50240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18628145 of line 449G02 Chr3:18628145
   
Insertion allele amplicon 8474 - 20dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20dr: 5'-GCTTCTTATGGAGCATGGGTATAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18627613
   
wt amplicon (over InsPos) 56dy - 20dr
wt amplicon start Chr3:18627613
wt amplicon end Chr3:18628761
wt amplicon size 1149 bp
Locus specific primer 20dr: 5'-GCTTCTTATGGAGCATGGGTATAG-3'
2nd locus specific primer 56dy: 5'-GTCCTCTTCTTCTTCTTCTCCTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11