Genotyping and primer details


Genotyping and primer details for 453G07 - At1g13440
Line ID 453G07
Locus At1g13440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4610218 of line 453G07 Chr1:4610218
   
Insertion allele amplicon 8474 - HM67
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HM67: 5'-GGTTCCTTCAAAAATAAAACACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4609764
   
wt amplicon (over InsPos) 03cm - HM67
wt amplicon start Chr1:4609764
wt amplicon end Chr1:4610647
wt amplicon size 884 bp
Locus specific primer HM67: 5'-GGTTCCTTCAAAAATAAAACACCA-3'
2nd locus specific primer 03cm: 5'-GCCACTCGATCATATTTTCAATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11