Genotyping and primer details


Genotyping and primer details for 454E07 - At5g57710
Line ID 454E07
Locus At5g57710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23386222 of line 454E07 Chr5:23386222
   
Insertion allele amplicon 8474 - 24uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 24uj: 5'-GGACTCATAGGCTTCAAGTGTACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23386597
   
wt amplicon (over InsPos) 36vu - 24uj
wt amplicon start Chr5:23385607
wt amplicon end Chr5:23386597
wt amplicon size 991 bp
Locus specific primer 24uj: 5'-GGACTCATAGGCTTCAAGTGTACC-3'
2nd locus specific primer 36vu: 5'-GAGGAGATTAGTTCCGATAAGGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11