Genotyping and primer details


Genotyping and primer details for 455G05 - At5g14550
Line ID 455G05
Locus At5g14550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4692739 of line 455G05 Chr5:4692739
   
Insertion allele amplicon 8474 - 17nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17nt: 5'-ACCGCTTTGTTTTTCTTTCTGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4693104
   
wt amplicon (over InsPos) 01oe - 17nt
wt amplicon start Chr5:4692387
wt amplicon end Chr5:4693104
wt amplicon size 718 bp
Locus specific primer 17nt: 5'-ACCGCTTTGTTTTTCTTTCTGATA-3'
2nd locus specific primer 01oe: 5'-TAAGTCTCTCTTGGCAGCTAAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11