Genotyping and primer details


Genotyping and primer details for 456H02 - At3g19830
Line ID 456H02
Locus At3g19830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6888758 of line 456H02 Chr3:6888758
   
Insertion allele amplicon 8474 - 25uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25uj: 5'-GAAGTTTCTGGGCATTTACAAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6888196
   
wt amplicon (over InsPos) 49vc - 25uj
wt amplicon start Chr3:6888196
wt amplicon end Chr3:6889323
wt amplicon size 1128 bp
Locus specific primer 25uj: 5'-GAAGTTTCTGGGCATTTACAAGAG-3'
2nd locus specific primer 49vc: 5'-TATAGAGGTGGGATTGAGAATTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11