Genotyping and primer details


Genotyping and primer details for 460E02 - At5g14610
Line ID 460E02
Locus At5g14610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4713487 of line 460E02 Chr5:4713487
   
Insertion allele amplicon 8474 - 41ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 41ve: 5'-GCGATGCAAAACAGAGACATAGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4713006
   
wt amplicon (over InsPos) 40vw - 41ve
wt amplicon start Chr5:4713006
wt amplicon end Chr5:4714146
wt amplicon size 1141 bp
Locus specific primer 41ve: 5'-GCGATGCAAAACAGAGACATAGTA-3'
2nd locus specific primer 40vw: 5'-CCATTTGGAAAATCATAGTTGACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11