Genotyping and primer details


Genotyping and primer details for 462A04 - At5g55550
Line ID 462A04
Locus At5g55550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22502798 of line 462A04 Chr5:22502798
   
Insertion allele amplicon 8474 - NU29
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NU29: 5'-CCATTGTAAGTTTCTGGGTCATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22502220
   
wt amplicon (over InsPos) 53rd - NU29
wt amplicon start Chr5:22502220
wt amplicon end Chr5:22503242
wt amplicon size 1023 bp
Locus specific primer NU29: 5'-CCATTGTAAGTTTCTGGGTCATTT-3'
2nd locus specific primer 53rd: 5'-CGCTAGTCCTATCCACCTTATGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11