Genotyping and primer details


Genotyping and primer details for 464A10 - At5g41800
Line ID 464A10
Locus At5g41800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16734151 of line 464A10 Chr5:16734151
   
Insertion allele amplicon 8474 - 43vd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 43vd: 5'-ATTCCAGTGTTGATAGCTGTTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16734558
   
wt amplicon (over InsPos) 22vp - 43vd
wt amplicon start Chr5:16733661
wt amplicon end Chr5:16734558
wt amplicon size 898 bp
Locus specific primer 43vd: 5'-ATTCCAGTGTTGATAGCTGTTTGA-3'
2nd locus specific primer 22vp: 5'-TCCTTTTCTCACTATGACAGGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11