Genotyping and primer details


Genotyping and primer details for 466C11 - At2g41180
Line ID 466C11
Locus At2g41180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17165622 of line 466C11 Chr2:17165622
   
Insertion allele amplicon 8474 - 68lx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 68lx: 5'-CTAACTCATGTGTTTAGAACAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17166482
   
wt amplicon (over InsPos) 38rz - 68lx
wt amplicon start Chr2:17165272
wt amplicon end Chr2:17166482
wt amplicon size 1211 bp
Locus specific primer 68lx: 5'-CTAACTCATGTGTTTAGAACAAA-3'
2nd locus specific primer 38rz: 5'-AACCAGAGAAAGTCATCATCGTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11