Genotyping and primer details


Genotyping and primer details for 470E02 - At3g15605
Line ID 470E02
Locus At3g15605 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5290819 of line 470E02 Chr3:5290819
   
Insertion allele amplicon 8474 - 30aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30aq: 5'-ATTGAGAACCTGGAACCATCATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5290083
   
wt amplicon (over InsPos) 18ax - 30aq
wt amplicon start Chr3:5290083
wt amplicon end Chr3:5290917
wt amplicon size 835 bp
Locus specific primer 30aq: 5'-ATTGAGAACCTGGAACCATCATAC-3'
2nd locus specific primer 18ax: 5'-GAATTTATGGGGTCTGTTTACGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11