Genotyping and primer details


Genotyping and primer details for 472C01 - At3g09600
Line ID 472C01
Locus At3g09600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2947670 of line 472C01 Chr3:2947670
   
Insertion allele amplicon 8474 - 54ns
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 54ns: 5'-TAAAAGAAGTGCCTCCAACTCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2948082
   
wt amplicon (over InsPos) 17oj - 54ns
wt amplicon start Chr3:2947163
wt amplicon end Chr3:2948082
wt amplicon size 920 bp
Locus specific primer 54ns: 5'-TAAAAGAAGTGCCTCCAACTCATT-3'
2nd locus specific primer 17oj: 5'-TATCCTCAAAAGGCATCAAAGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11