Genotyping and primer details


Genotyping and primer details for 473G06 - At5g10370
Line ID 473G06
Locus At5g10370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3263001 of line 473G06 Chr5:3263001
   
Insertion allele amplicon 8474 - 16vc
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16vc: 5'-AAATTAACTTTCCAAGCTTTGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3263671
   
wt amplicon (over InsPos) 23vl - 16vc
wt amplicon start Chr5:3262812
wt amplicon end Chr5:3263671
wt amplicon size 860 bp
Locus specific primer 16vc: 5'-AAATTAACTTTCCAAGCTTTGGCT-3'
2nd locus specific primer 23vl: 5'-GATTGACCTGAGGCTTGTAATCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11