Genotyping and primer details


Genotyping and primer details for 476C04 - At5g65400
Line ID 476C04
Locus At5g65400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26132081 of line 476C04 Chr5:26132081
   
Insertion allele amplicon 8474 - 41uw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 41uw: 5'-AATTTTATGCCTTCATGGATTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26131540
   
wt amplicon (over InsPos) 74vo - 41uw
wt amplicon start Chr5:26131540
wt amplicon end Chr5:26132368
wt amplicon size 829 bp
Locus specific primer 41uw: 5'-AATTTTATGCCTTCATGGATTCAG-3'
2nd locus specific primer 74vo: 5'-TTAGTTAGAGCCGATCCCTACAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11