Genotyping and primer details


Genotyping and primer details for 477H04 - At1g13050
Line ID 477H04
Locus At1g13050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4451392 of line 477H04 Chr1:4451392
   
Insertion allele amplicon 8474 - 49kq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 49kq: 5'-ATTCTCCTCTTAGTCTACCTCGCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4451027
   
wt amplicon (over InsPos) 12lb - 49kq
wt amplicon start Chr1:4451027
wt amplicon end Chr1:4451874
wt amplicon size 848 bp
Locus specific primer 49kq: 5'-ATTCTCCTCTTAGTCTACCTCGCC-3'
2nd locus specific primer 12lb: 5'-CTTTTCGTGTAACATGCACAAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11