Genotyping and primer details


Genotyping and primer details for 478A02 - At1g50280
Line ID 478A02
Locus At1g50280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18624627 of line 478A02 Chr1:18624627
   
Insertion allele amplicon 8474 - 33cy
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 33cy: 5'-TCGACATATCTCTGTTCGATCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18624145
   
wt amplicon (over InsPos) 58dy - 33cy
wt amplicon start Chr1:18624145
wt amplicon end Chr1:18625064
wt amplicon size 920 bp
Locus specific primer 33cy: 5'-TCGACATATCTCTGTTCGATCTTG-3'
2nd locus specific primer 58dy: 5'-AATTTGAAAGTCCCCAAGTTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11