Genotyping and primer details


Genotyping and primer details for 478H10 - At1g67280
Line ID 478H10
Locus At1g67280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25189860 of line 478H10 Chr1:25189860
   
Insertion allele amplicon 8474 - 17wu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17wu: 5'-AGATGTTCCTCAATCACAGCTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25190285
   
wt amplicon (over InsPos) 22xd - 17wu
wt amplicon start Chr1:25189032
wt amplicon end Chr1:25190285
wt amplicon size 1254 bp
Locus specific primer 17wu: 5'-AGATGTTCCTCAATCACAGCTTTT-3'
2nd locus specific primer 22xd: 5'-TGTACTGCAAATAACCACTTACGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11